Group

Detected viruses

Limits of detection (copies/UL)

Single plex qRT-PCR assays

Duplexq RT-PCR assays

RT-PCR assays

A

EEEV

133.5

114.4

114,400

WEEV

155.7

215.6

215,600

B

VEEV

94.3

134.8

134,800

CHIKV

103.5

146

146

C

MVEV

114.2

124.8

124,800

SLEV

116.9

110.4

1104

D

WNV

105.6

116.5

11,650

JEV

100.1

174.3

174,300

E

CEV

143.9

143.5

14,350

LCV

85.7

94.1

9410

F

POWV

86.3

124.3

12,430

TBEV

90.4

103.5

10,350

G

TOSV

78.1

107.1

10,710

RVFV

101.3

110.6

1106

H

DENV

110.8

131.2

131,200