| Group | Detected viruses | Limits of detection (copies/UL) | ||
| Single plex qRT-PCR assays | Duplexq RT-PCR assays | RT-PCR assays | ||
| A | EEEV | 133.5 | 114.4 | 114,400 |
| WEEV | 155.7 | 215.6 | 215,600 | |
| B | VEEV | 94.3 | 134.8 | 134,800 |
| CHIKV | 103.5 | 146 | 146 | |
| C | MVEV | 114.2 | 124.8 | 124,800 |
| SLEV | 116.9 | 110.4 | 1104 | |
| D | WNV | 105.6 | 116.5 | 11,650 |
| JEV | 100.1 | 174.3 | 174,300 | |
| E | CEV | 143.9 | 143.5 | 14,350 |
| LCV | 85.7 | 94.1 | 9410 | |
| F | POWV | 86.3 | 124.3 | 12,430 |
| TBEV | 90.4 | 103.5 | 10,350 | |
| G | TOSV | 78.1 | 107.1 | 10,710 |
| RVFV | 101.3 | 110.6 | 1106 | |
| H | DENV | 110.8 | 131.2 | 131,200 |