Assays | RNA transcripts | RNA transcripts concentration | Mean Ct value | Intra-assay CV (%) | Inter-assay CV (%) | linear range |
Group A | EEEV | 107 copies/µL | 18.13 | 1.96 | 3.41 | Y = 3.46x + 46.25 R2 = 0.996 |
106 copies/µL | 22.06 | 2.17 | 2.01 | |||
105 copies/µL | 25.22 | 1.22 | 1.59 | |||
104 copies/µL | 29.31 | 1.05 | 0.51 | |||
103 copies/µL | 33.17 | 1.29 | 0.3 | |||
WEEV | 107 copies/µL | 20.02 | 4.81 | 4.68 | Y = 3.68x + 47.69 R2 = 0.988 | |
106 copies/µL | 22.96 | 3.28 | 0.56 | |||
105 copies/µL | 26.14 | 1.47 | 0.82 | |||
104 copies/µL | 30.11 | 1.3 | 0.4 | |||
103 copies/µL | 34.15 | 0.95 | 0.71 | |||
Group B | VEEV | 107 copies/µL | 17.51 | 4.46 | 1.52 | Y = 3.66x + 47.2 R2 = 0.998 |
106 copies/µL | 21.04 | 3.3 | 0.21 | |||
105 copies/µL | 24.16 | 2.77 | 0.58 | |||
104 copies/µL | 27.20 | 0.69 | 0.44 | |||
103 copies/µL | 30.43 | 1.7 | 0.59 | |||
CHIKV | 107 copies/µL | 19.65 | 0.59 | 0.59 | Y = 3.58x + 47.49 R2 = 0.999 | |
106 copies/µL | 22.14 | 2.74 | 2.74 | |||
105 copies/µL | 25.63 | 0.73 | 0.73 | |||
104 copies/µL | 29.07 | 0.37 | 0.37 | |||
103 copies/µL | 33.12 | 0.14 | 0.14 | |||
Group C | MVEV | 107 copies/µL | 14.82 | 2.87 | 1.27 | |
106 copies/µL | 18.31 | 1.39 | 1.64 | Y = 3.53x + 46.31 R2 = 0.998 | ||
105 copies/µL | 23.85 | 0.73 | 1.72 | |||
104 copies/µL | 28.02 | 0.79 | 1.32 | |||
103 copies/µL | 32.97 | 1.17 | 0.68 | |||
SLEV | 107 copies/µL | 14.57 | 2.82 | 0.98 | Y = 3.56x + 46.69 R2 = 0.997 | |
106 copies/µL | 18.61 | 3.08 | 4.06 | |||
105 copies/µL | 24.03 | 1.97 | 0.9 | |||
104 copies/µL | 28.09 | 1.03 | 0.68 | |||
103 copies/µL | 33.11 | 2.15 | 0.81 | |||
Group D | WNV | 107 copies/µL | 14.61 | 2.2 | 3.79 | Y = 3.54x + 47.26 R2 = 0.997 |
106 copies/µL | 19.58 | 1.16 | 0.37 | |||
105 copies/µL | 24.64 | 0.14 | 0.21 |