Assays

RNA transcripts

RNA transcripts concentration

Mean Ct value

Intra-assay CV (%)

Inter-assay CV (%)

linear range

Group A

EEEV

107 copies/µL

18.13

1.96

3.41

Y = 3.46x + 46.25

R2 = 0.996

106 copies/µL

22.06

2.17

2.01

105 copies/µL

25.22

1.22

1.59

104 copies/µL

29.31

1.05

0.51

103 copies/µL

33.17

1.29

0.3

WEEV

107 copies/µL

20.02

4.81

4.68

Y = 3.68x + 47.69

R2 = 0.988

106 copies/µL

22.96

3.28

0.56

105 copies/µL

26.14

1.47

0.82

104 copies/µL

30.11

1.3

0.4

103 copies/µL

34.15

0.95

0.71

Group B

VEEV

107 copies/µL

17.51

4.46

1.52

Y = 3.66x + 47.2

R2 = 0.998

106 copies/µL

21.04

3.3

0.21

105 copies/µL

24.16

2.77

0.58

104 copies/µL

27.20

0.69

0.44

103 copies/µL

30.43

1.7

0.59

CHIKV

107 copies/µL

19.65

0.59

0.59

Y = 3.58x + 47.49

R2 = 0.999

106 copies/µL

22.14

2.74

2.74

105 copies/µL

25.63

0.73

0.73

104 copies/µL

29.07

0.37

0.37

103 copies/µL

33.12

0.14

0.14

Group C

MVEV

107 copies/µL

14.82

2.87

1.27

106 copies/µL

18.31

1.39

1.64

Y = 3.53x + 46.31

R2 = 0.998

105 copies/µL

23.85

0.73

1.72

104 copies/µL

28.02

0.79

1.32

103 copies/µL

32.97

1.17

0.68

SLEV

107 copies/µL

14.57

2.82

0.98

Y = 3.56x + 46.69

R2 = 0.997

106 copies/µL

18.61

3.08

4.06

105 copies/µL

24.03

1.97

0.9

104 copies/µL

28.09

1.03

0.68

103 copies/µL

33.11

2.15

0.81

Group D

WNV

107 copies/µL

14.61

2.2

3.79

Y = 3.54x + 47.26

R2 = 0.997

106 copies/µL

19.58

1.16

0.37

105 copies/µL

24.64

0.14

0.21