Group

Viruses

GenBank\Accession No.

Forward Primers/Tm value

Reverse Primers/Tm value

Probes/Tm value

Ampliconsize (bp)

A

EEEV

NC_003899.1

CTGTGTGTTCGTACGCTGCG/60.1

GCTGCTTATTTTGCTGTGGGC/60/7

CGCCCAAGGCGCCGCAGACAA/74.7

75

WEEV

NC_003908.1

GATCGGGCCGTCCATGAG/61

GCTTCTATTTCCTTCAGAGGCG/58.3

TACGCCCCGCGCCTCGATC/69.3

105

B

VEEV

NC_001449.1

CCCCGTTCAATGTGTCTGTCAC/61.2

CAGGCTATGCTGCTACGATGC/59.6

TTGCAGCACAAGAATCCCTCGCG/69.1

69

CHIKV

NC_004162.2

TGGCTTTTTTAGCCGTAATGAGC/60.5

CGGTACTCCCACCGTGTTCG/62.2

TCGGTGCCCACACTGTGAGCGC/71.5

88

C

MVEV

NC_000943.1

GCCATGATGGTGATGCAACT/58

CTGTCTGGGAATGAGCAGCC/59.4

TCGCCCTCCAGCACCAAATCGA/69.6

99

SLEV

NC_007580.2

CTTGTGCGTCCTCTCCAGCC/61.8

CTGGGTGCAAAGCCCCTC/60.2

CGTGCCAGGGACCCTCCCGAGTC/72.4

68

D

WNV

NC_001563.2

CAGCGATCTCTCCACCAAAGC/60.8

GGGTCAGCACGTTTGTCATTG/60

TGCCCGACCATGGGAGAAGCTCA/70.6

69

JEV

NC_001437.1

ACTGGGTTACCAAAGCCGTTG/59.9

AGTCCTTCCACCTCCTCTACAGC/58.9

CCCCCACGGCCCAAGCCTCGT/73.5

152

E

CEV

U12800.1

AGCAGGATATAGGTCATTTCTGCC/58.7

GCCAATCGCAGTTGCTTATATG/58.4

CCCCAGGTGTGCCACTGTTAGATTCC/68.1

90

LCV

NC_004109.1

ATACACACCCATCACTTACAGCC/56.7

CATTTGCAAGAGAGAGGACAAGC/ CATTTGCAAGAGAGAGGACAAGT/59.1

AGGCAACCAAACTCTTCGCATCCCC/69.5

75

F

POWV

NC_003687.1

GGCACTCCCCAACTCCG/ GGCACTCCCCAGCTCCA/59.3

GCTGGGGCAAGTCAATCTTG/59.4

TCAACCCCCATCATCATGCGCCT/70.1

81

TBEV

NC_001672.1

GGGGGGCGGTTCTTGTT/59.3

CACACATCACCTCCTTGTCAGAC/58.2

CTCCCTGAGCCACCATCACCCAGAC/69.6

72

G

TOSV

NC_006320.1

CTAACTGGGCCACACACATGC/60

TCACCATTGCTCGCACTGG/60.4

CTGCCTATTCCCCCCCTAACCCC/67

135

RVFV

NC_014396.1

CTTGACCCCCTTCAACATCAAA/59.8

CTCCAGAATCACCACTTGCTCTAC/58.1

AAGCCTCTGCCCCAACTGACCCTGC/71.6

121

H

DENV

NC_001474.1

CAAAAGGAAGTCGYGCAATA/53.8

CTGAGTGAATTCTCTCTGCTRAAC/56.5

CATGTGGYTGGGAGCRCGC/65.5

112

IC

HM745932

GTCAAGATCCTCAAAGATACAGCT/54.8

ACTCTTGGCCGTTGGTTTG/57.3

AGTTTGGAGTCTTGGATGTCGCAT/62

113