Reagents | Volume |
2X One-Step RT-ddRT-PCR supermix | 10 μL |
25 mM manganese acetate solution | 0.8 μL |
RT-PCR Forward Primer (10 uM) | 0.4 μL |
RT-PCR Reverse Primer (10 uM) | 0.4 μL |
TaqMan Probe | 0.4 μL |
DEPC H2O | 6 μL |
RNA | 2 μL |
Total | 20 μL |