Reagents

Volume

2X One-Step RT-ddRT-PCR supermix

10 μL

25 mM manganese acetate solution

0.8 μL

RT-PCR Forward Primer (10 uM)

0.4 μL

RT-PCR Reverse Primer (10 uM)

0.4 μL

TaqMan Probe

0.4 μL

DEPC H2O

6 μL

RNA

2 μL

Total

20 μL