| Reagents | Volume |
| 2X One-Step RT-ddRT-PCR supermix | 10 μL |
| 25 mM manganese acetate solution | 0.8 μL |
| RT-PCR Forward Primer (10 uM) | 0.4 μL |
| RT-PCR Reverse Primer (10 uM) | 0.4 μL |
| TaqMan Probe | 0.4 μL |
| DEPC H2O | 6 μL |
| RNA | 2 μL |
| Total | 20 μL |