| Method | Composition of 1 L simulate gastric solution | pH | Incubation time |
| PBET | 1.25 g pepsin, 0.5 g citrate, 0.5 g malate, 0.42 ml lactic acid and 0.5 ml acetic acid | adjusted to 2.5 with 1:1 HCl | 1 h |
| USPM | 3.20 g pepsin, 7.0 ml concentrated HCl and 2.0 g NaCl | not strictly controlled, the theoretical pH was 1.08 | 2 h |