Step

Reagent

Time

1.

95% Alcohol (Fixation)

15 - 30 minutes

2.

60% Alcohol

2 minutes

3.

Distilled Water

5 dips

4.

Distilled Water

5 dips

5.

Hematoxylin stain

3 minutes

6.

Distilled Water

3 minutes

7.

60% Alcohol

2 minutes

8.

80% Alcohol

2 minutes

9.

95% Alcohol

2 minutes

10.

Orange G Stain

3 minutes

11.

95% ALcohol

2 minutes

12.

95% Alcohol

2 minutes

13.

Eosin Azure Stain

3 minutes

14.

95% Alcohol

2 minutes

15.

95% Alcohol

2 minutes

16.

95% Alcohol

2 minutes

17.

95% Alcohol

2 minutes

18.

Absolute Alcohol

2 minutes

19.

Absolute Alcohol

2 minutes

20.

Absolute Alcohol

2 minutes

21.

Absolute Alcohol + Xylene (1:1)

2 minutes

22.

Xylene

2 minutes

23.

Xylene

2 minutes

24.

Xylene

Till clear

25.

Mount in D.P.X