Step | Reagent | Time |
1. | 95% Alcohol (Fixation) | 15 - 30 minutes |
2. | 60% Alcohol | 2 minutes |
3. | Distilled Water | 5 dips |
4. | Distilled Water | 5 dips |
5. | Hematoxylin stain | 3 minutes |
6. | Distilled Water | 3 minutes |
7. | 60% Alcohol | 2 minutes |
8. | 80% Alcohol | 2 minutes |
9. | 95% Alcohol | 2 minutes |
10. | Orange G Stain | 3 minutes |
11. | 95% ALcohol | 2 minutes |
12. | 95% Alcohol | 2 minutes |
13. | Eosin Azure Stain | 3 minutes |
14. | 95% Alcohol | 2 minutes |
15. | 95% Alcohol | 2 minutes |
16. | 95% Alcohol | 2 minutes |
17. | 95% Alcohol | 2 minutes |
18. | Absolute Alcohol | 2 minutes |
19. | Absolute Alcohol | 2 minutes |
20. | Absolute Alcohol | 2 minutes |
21. | Absolute Alcohol + Xylene (1:1) | 2 minutes |
22. | Xylene | 2 minutes |
23. | Xylene | 2 minutes |
24. | Xylene | Till clear |
25. | Mount in D.P.X |
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