Micro-organisms

Incubation

Culture medium

Volume

Analysis method

Observations

References

Total coliforms

37˚C for 24 h

Lactose agar with TTC and at the Tergito

100 mL

Filtration technique

on 0.45 μm filter membrane

Red colony

NF EN ISO 9308-3

(1999)

Faecal coliforms

44˚C from 18 à 24 h

Lactose agar with TTC and at the Tergito

100 mL

Filtration technique

on 0.45 μm filter membrane

Red colony

NF EN ISO 9308-3 (1999)

Faecal streptococci

37˚C/48h

Slanetz-Bartley

100 mL

Filtration technique

on 0.45 μm filter membrane

Red colony

NF ISO 7899-2

Staphylococci

37˚C 24 - 48 h

Chapman

100 mL

Filtration technique

on 0.45 μm filter membrane

Red colony

NF T90-412

Clostridium

24 - 48 h

SPS/TSN

20 mL

Incorporation into agar

Black halo of significant size

NF T 90-415

Yeasts

5 days

Sabouraud with chloramphenicol

100 mL

Filtration technique

on 0.45 μm filter membrane

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