| Reason for refusal | Effective | % |
| Fear of divorce so positivity | 4 | 11.11 |
| Get the partner’s opinion on the test | 18 | 50 |
| Think you’re healthy | 2 | 5.56 |
| Fear of disease | 10 | 27.78 |
| No motive | 2 | 5.55 |
| Total | 36 | 100 |