Gene region | Reaction No. | Primers | Different haplotypes/ genotypes | PCR product lengths |
Promoter | 1 | L: 5′CTTACCCAGGCAAGCCGGTC3′ + X: 5′GCTGTCTACAAATATCAGAAGGTC3′ | LX haplotype | 373 bp |
2 | L: 5′CTTACCCAGGCAAGCCGGTC3′ + Y: 5′CCTGTCTACAAATATCAGAAGGTC 3′ | LY haplotype | ||
3 | H: 5′CTTACCCAGGCAAGCCGGTG3′ + Y: 5′CCTGTCTACAAATATCAGAAGGTC 3′ | HY haplotype | ||
1st exon | 4 | CF: 5′ GCAGCGTCTTACTCAGAAACTGTG3′ + CR: 3′GGGCTGGCAAGACAACTATTAGTC5′ + 52R-D: 3′ACAGTACCGTGGTTCCCTCT5′ + 54R-B: 3′TGTTGTTCCCTCTTTTCCCC5′ + 57R-C: 3′TTCGTTTCCCCCTTGGTCG5′ | D/A or D/D | 128 + 339 bp |
B/A or B/B | 135 + 339 bp | |||
C/A or C/C | 143 + 339 bp | |||
D/B genotype | 128 + 135 + 339 bp | |||
D/C genotype | 128 + 143 + 339 bp | |||
B/C genotype | 135 + 143 + 339 bp | |||
A/A genotype | 339 | |||
5 | CF + CR + 52R-ABC: 3′GCAGTACCGTGGTTCCCTCT5′ + 54R-ACD: 3′ CGTTGTTCCCTCTTTCCCC5′ + 57R-ABD: 3′CTCGTTTCCCCCTTGGTCG5′ | D/D genotype | 135 + 143 + 339 bp | |
B/B genotype | 128 + 143 + 339 bp | |||
C/C genotype | 128 + 135 + 339 bp | |||
A/A, A/B, A/C or A/D genotype | 128 + 135 + 143 + 339 bp |