Gene

region

Reaction

No.

Primers

Different haplotypes/

genotypes

PCR product lengths

Promoter

1

L: 5′CTTACCCAGGCAAGCCGGTC3′

+

X: 5′GCTGTCTACAAATATCAGAAGGTC3′

LX haplotype

373 bp

2

L: 5′CTTACCCAGGCAAGCCGGTC3′

+

Y: 5′CCTGTCTACAAATATCAGAAGGTC 3′

LY haplotype

3

H: 5′CTTACCCAGGCAAGCCGGTG3′

+

Y: 5′CCTGTCTACAAATATCAGAAGGTC 3′

HY haplotype

1st exon

4

CF: 5′ GCAGCGTCTTACTCAGAAACTGTG3′

+ CR: 3′GGGCTGGCAAGACAACTATTAGTC5′

+ 52R-D: 3′ACAGTACCGTGGTTCCCTCT5′

+ 54R-B: 3′TGTTGTTCCCTCTTTTCCCC5′

+ 57R-C: 3′TTCGTTTCCCCCTTGGTCG5′

D/A or D/D

128 + 339 bp

B/A or B/B

135 + 339 bp

C/A or C/C

143 + 339 bp

D/B genotype

128 + 135 + 339 bp

D/C genotype

128 + 143 + 339 bp

B/C genotype

135 + 143 + 339 bp

A/A genotype

339

5

CF + CR +

52R-ABC: 3′GCAGTACCGTGGTTCCCTCT5′

+ 54R-ACD: 3′ CGTTGTTCCCTCTTTCCCC5′

+ 57R-ABD: 3′CTCGTTTCCCCCTTGGTCG5′

D/D genotype

135 + 143 + 339 bp

B/B genotype

128 + 143 + 339 bp

C/C genotype

128 + 135 + 339 bp

A/A, A/B, A/C or

A/D genotype

128 + 135 + 143 + 339 bp