| Gene and Accession Number | Primer | Amplicon Size |
| pKISS1 (NM_001134964.1)a |
|
|
| Forward | 5’-GGCAGCTGATGTTCTTTCTTTG-3’ | 89 bp |
| Reverse | 5’-CGGGCCTGTAGATCTAGGATT-3’ | |
| pKISS1R (NM_001044624.2)b |
|
|
| Forward | 5’-CAGGGAACTCACTTGTCATCTT-3’ | 110 bp |
| Reverse | 5’-GCACAGCAGAAACGTCAAATC-3’ | |
| pER-β (NM_001001533)c |
|
|
| Forward | 5’-GGACAGGGATGAAGGGAAATG-3’ | 124 bp |
| Reverse | 5’-CATGGCCTTGACACAGAGATAC-3’ | |
| pER-α (NM_214220)d |
|
|
| Forward | 5’-GAATGTTGAAGCACAAGCGCCAGA-3’ | 91 bp |
| Reverse | 5’-ACCGGGCTGTTCTTCTTAGTGTGT-3’ | |
| pGnRHR (AF017079.1)f |
|
|
| Forward | 5’-AGCCAACCTGTTGGAGACTCTGAT -3’ | 101 bp |
| Reverse | 5’-AGCTGAGGACTTTGCAGAGGAACT-3’ | |
| pGAPDH (AF017079.1)f |
|
|
| Forward | 5’-GCAAAGTGGACATTGTCGCCATCA-3’ | 105 bp |
| Reverse | 5’-TGACTGTGCCGTGGAATTTGCCAT-3’ | |
| pβ-actin (U07786.1)g |
|
|
| Forward | 5’-TCGCCGACAGGATGCAGAAGGA-3’ | 129 bp |
| Reverse | 5’-AGGTGGACAGCGAGGCCAGGAT-3 |