Enzymes | pH | Km (mM) | Kcat (s−1) | Kcat/Km (s−1·mM−1) | pH | Km (mM) | Kcat (s−1) | Kcat/Km (s−1·mM−1) |
MnP III (WT) | 5.0 | 0.17 ± 10 | 175 ± 3.0 | 1029.41 | 8.0 | 0.011 ± 0.0001 | 34.2 ± 0.8 | 3109.1 |
E40H | 5.0 | 11.1 ± 0.4 | 12.0 ± 0.2 | 1.01 | 8.0 | 0.222 ± 0.03 | 20.5 ± 0.7 | 92.3 |
E44H | 5.0 | 8.5 ± 0.5 | 12.1 ± 0.3 | 1.49 | 8.0 | 0.124 ± 0.01 | 30.2 ± 0.4 | 243.6 |
E40H/E44H | 5.0 | 19.9 ± 2.5 | 1. 7 ± 0.1 | 0.08 | 8.0 | 1.086 ± 0.10 | 31.7 ± 1.5 | 29.2 |
D189H | 5.0 | 23.1 ± 3.9 | 0.84 ± 0.1 | 0.04 | 8.0 | 0.42 ± 0.05 | 53.0 ± 1.2 | 128.0 |
D189N | 5.0 | 30.3 ± 3.7 | 8.0 ± 0.5 | 0.30 | 8.0 | 0.33 ± 0.02 | 5.0 ± 0.1 | 15.2 |