Enzymes

pH

Km

(mM)

Kcat

(s−1)

Kcat/Km

(s−1·mM−1)

pH

Km

(mM)

Kcat

(s−1)

Kcat/Km

(s−1·mM−1)

MnP III (WT)

5.0

0.17 ± 10

175 ± 3.0

1029.41

8.0

0.011 ± 0.0001

34.2 ± 0.8

3109.1

E40H

5.0

11.1 ± 0.4

12.0 ± 0.2

1.01

8.0

0.222 ± 0.03

20.5 ± 0.7

92.3

E44H

5.0

8.5 ± 0.5

12.1 ± 0.3

1.49

8.0

0.124 ± 0.01

30.2 ± 0.4

243.6

E40H/E44H

5.0

19.9 ± 2.5

1. 7 ± 0.1

0.08

8.0

1.086 ± 0.10

31.7 ± 1.5

29.2

D189H

5.0

23.1 ± 3.9

0.84 ± 0.1

0.04

8.0

0.42 ± 0.05

53.0 ± 1.2

128.0

D189N

5.0

30.3 ± 3.7

8.0 ± 0.5

0.30

8.0

0.33 ± 0.02

5.0 ± 0.1

15.2