| Primer | Target gene | Sequence (Forward/Reverse) | Amplified fragment size (bp) | Annealing Temp |
| Bla SHV | ESBL | 5’TGGTTATGCGTTATATTCGCC3’ 5’GGTTAGCGTTGCCAGTGCT-3’ | 868 | 56 |
| Bla TEM | ESBL | 5’TCCGTCATGAGACAATAACC-3’ 5’TTGGTCTGACAGTTACCAATGC-3’ | 972 | 56 |
| Bla CTX-M1 | ESBL | 5’AAAAATCACTGCGCCAGTTC3’ 5’AGCTTATTCATCGCCACGTT3’ | 415 | 56 |