Stain

Conc.

Staining percent

1 h

2 h

4 h

8 h

Acridine Orange

5 µg/ml

80.98 ± 2.02

83.20 ± 1.38

87.61 ± 1.12

87.93 ± 1.10

10 µg/ml

90.82 ± 0.56

98.24 ± 0.16

99.45 ± 0.39

99.56 ± 0.31

25 µg/ml

92.84 ± 0.73

98.79 ± 0.45

100.00 ± 00

100.00 ± 00

50 µg/ml

93.73 ± 0.48

99.05 ± 0.67

100.00 ± 00

100.00 ± 00

LDS 751

5 µg/ml

44.30 ± 2.29

52.19 ± 2.71

61.47 ± 3.51

59.01 ± 2.60

10 µg/ml

54.62 ± 3.40

80.85 ± 1.63

89.94 ± 2.23

83.63 ± 1.06

25 µg/ml

64.45 ± 2.31

90.29 ± 0.49

98.93 ± 0.76

97.84 ± 0.94

50 µg/ml

71.56 ± 2.01

91.45 ± 1.27

98.45 ± 0.86

98.10 ± 1.13

SYTO Orange 81

5 µM

20.94 ± 2.28

24.03 ± 2.38

25.63 ± 4.23

22.06 ± 2.35

10 µM

33.35 ± 1.92

51.90 ± 2.03

59.09 ± 3.56

54.62 ± 1.71

25 µM

48.23 ± 2.32

72.69 ± 1.54

81.15 ± 1.75

78.72 ± 1.69

50 µM

54.80 ± 2.69

76.26 ± 2.56

88.83 ± 1.00

85.90 ± 1.21

SYTO Orange 82

5 µM

21.28 ± 2.08

21.26 ± 2.89

19.76 ± 1.95

20.38 ± 2.54

10 µM

31.20 ± 2.00

46.65 ± 1.89

56.16 ± 4.09

64.68 ± 1.86

25 µM

47.02 ± 1.06

70.67 ± 1.64

81.42 ± 1.34

73.73 ± 1.39

50 µM

55.22 ± 1.22

81.24 ± 1.20

89.92 ± 0.85

85.77 ± 1.54

SYTO Orange 83

5 µM

35.24 ± 2.94

40.64 ± 2.29

39.91 ± 1.60

40.41 ± 1.65

10 µM

60.08 ± 1.50

72.85 ± 1.60

77.16 ± 1.19

76.79 ± 1.70

25 µM

65.41 ± 1.97

80.99 ± 0.48

93.34 ± 1.82

92.95 ± 0.92

50 µM

71.85 ± 3.56

85.33 ± 2.26

93.43 ± 2.00

92.89 ± 1.60

SYTO Orange 84

5 µM

27.76 ± 2.17

27.60 ± 2.39

28.57 ± 2.86

27.57 ± 2.27

10 µM

41.69 ± 0.52

60.73 ± 1.52

60.47 ± 3.07

63.75 ± 1.69

25 µM

51.00 ± 3.67

79.69 ± 0.73

85.76 ± 1.81

82.85 ± 1.68

50 µM

68.08 ± 3.20

82.28 ± 1.07

88.93 ± 1.34

88.92 ± 2.49

SYTO Orange 85

5 µM

55.89 ± 2.02

58.38 ± 3.45

57.43 ± 2.09

59.86 ± 0.79

10 µM

73.66 ± 1.92

79.22 ± 1.95

82.17 ± 1.58

82.15 ± 0.62

25 µM

85.36 ± 1.23

88.29 ± 2.61

90.09 ± 2.01

89.20 ± 1.99

50 µM

86.28 ± 1.72

86.18 ± 0.87

92.28 ± 1.69

90.14 ± 1.72

SYTOX Green

5 µM

34.41 ± 1.62

36.17 ± 2.50

37.65 ± 0.85

39.62 ± 2.87

10 µM

58.67 ± 0.82

63.99 ± 3.24

66.33 ± 3.10

68.83 ± 3.08

25 µM

68.48 ± 1.46

76.40 ± 1.84

83.33 ± 1.43

77.80 ± 1.65

50 µM

73.43 ± 2.25

79.37 ± 1.52

83.23 ± 1.14

80.33 ± 2.99

Meldola’s Blue

0.05% w/v

26.04 ± 1.60

33.88 ± 1.77

42.08 ± 1.56

51.62 ± 1.43