Stain | Conc. | Staining percent | |||
1 h | 2 h | 4 h | 8 h | ||
Acridine Orange | 5 µg/ml | 80.98 ± 2.02 | 83.20 ± 1.38 | 87.61 ± 1.12 | 87.93 ± 1.10 |
10 µg/ml | 90.82 ± 0.56 | 98.24 ± 0.16 | 99.45 ± 0.39 | 99.56 ± 0.31 | |
25 µg/ml | 92.84 ± 0.73 | 98.79 ± 0.45 | 100.00 ± 00 | 100.00 ± 00 | |
50 µg/ml | 93.73 ± 0.48 | 99.05 ± 0.67 | 100.00 ± 00 | 100.00 ± 00 | |
LDS 751 | 5 µg/ml | 44.30 ± 2.29 | 52.19 ± 2.71 | 61.47 ± 3.51 | 59.01 ± 2.60 |
10 µg/ml | 54.62 ± 3.40 | 80.85 ± 1.63 | 89.94 ± 2.23 | 83.63 ± 1.06 | |
25 µg/ml | 64.45 ± 2.31 | 90.29 ± 0.49 | 98.93 ± 0.76 | 97.84 ± 0.94 | |
50 µg/ml | 71.56 ± 2.01 | 91.45 ± 1.27 | 98.45 ± 0.86 | 98.10 ± 1.13 | |
SYTO Orange 81 | 5 µM | 20.94 ± 2.28 | 24.03 ± 2.38 | 25.63 ± 4.23 | 22.06 ± 2.35 |
10 µM | 33.35 ± 1.92 | 51.90 ± 2.03 | 59.09 ± 3.56 | 54.62 ± 1.71 | |
25 µM | 48.23 ± 2.32 | 72.69 ± 1.54 | 81.15 ± 1.75 | 78.72 ± 1.69 | |
50 µM | 54.80 ± 2.69 | 76.26 ± 2.56 | 88.83 ± 1.00 | 85.90 ± 1.21 | |
SYTO Orange 82 | 5 µM | 21.28 ± 2.08 | 21.26 ± 2.89 | 19.76 ± 1.95 | 20.38 ± 2.54 |
10 µM | 31.20 ± 2.00 | 46.65 ± 1.89 | 56.16 ± 4.09 | 64.68 ± 1.86 | |
25 µM | 47.02 ± 1.06 | 70.67 ± 1.64 | 81.42 ± 1.34 | 73.73 ± 1.39 | |
50 µM | 55.22 ± 1.22 | 81.24 ± 1.20 | 89.92 ± 0.85 | 85.77 ± 1.54 | |
SYTO Orange 83 | 5 µM | 35.24 ± 2.94 | 40.64 ± 2.29 | 39.91 ± 1.60 | 40.41 ± 1.65 |
10 µM | 60.08 ± 1.50 | 72.85 ± 1.60 | 77.16 ± 1.19 | 76.79 ± 1.70 | |
25 µM | 65.41 ± 1.97 | 80.99 ± 0.48 | 93.34 ± 1.82 | 92.95 ± 0.92 | |
50 µM | 71.85 ± 3.56 | 85.33 ± 2.26 | 93.43 ± 2.00 | 92.89 ± 1.60 | |
SYTO Orange 84 | 5 µM | 27.76 ± 2.17 | 27.60 ± 2.39 | 28.57 ± 2.86 | 27.57 ± 2.27 |
10 µM | 41.69 ± 0.52 | 60.73 ± 1.52 | 60.47 ± 3.07 | 63.75 ± 1.69 | |
25 µM | 51.00 ± 3.67 | 79.69 ± 0.73 | 85.76 ± 1.81 | 82.85 ± 1.68 | |
50 µM | 68.08 ± 3.20 | 82.28 ± 1.07 | 88.93 ± 1.34 | 88.92 ± 2.49 | |
SYTO Orange 85 | 5 µM | 55.89 ± 2.02 | 58.38 ± 3.45 | 57.43 ± 2.09 | 59.86 ± 0.79 |
10 µM | 73.66 ± 1.92 | 79.22 ± 1.95 | 82.17 ± 1.58 | 82.15 ± 0.62 | |
25 µM | 85.36 ± 1.23 | 88.29 ± 2.61 | 90.09 ± 2.01 | 89.20 ± 1.99 | |
50 µM | 86.28 ± 1.72 | 86.18 ± 0.87 | 92.28 ± 1.69 | 90.14 ± 1.72 | |
SYTOX Green | 5 µM | 34.41 ± 1.62 | 36.17 ± 2.50 | 37.65 ± 0.85 | 39.62 ± 2.87 |
10 µM | 58.67 ± 0.82 | 63.99 ± 3.24 | 66.33 ± 3.10 | 68.83 ± 3.08 | |
25 µM | 68.48 ± 1.46 | 76.40 ± 1.84 | 83.33 ± 1.43 | 77.80 ± 1.65 | |
50 µM | 73.43 ± 2.25 | 79.37 ± 1.52 | 83.23 ± 1.14 | 80.33 ± 2.99 | |
Meldola’s Blue | 0.05% w/v | 26.04 ± 1.60 | 33.88 ± 1.77 | 42.08 ± 1.56 | 51.62 ± 1.43 |