Steps

Elute (ml)

Activity (iU/L)

Total activity (IU)

Protein concentration (mg/L)

Total protein (mg)

Specific activity (iU/mg)

Purification (folds

Yield%

Crude

7.5

73.02

0.547

51.4

0.386

1.419

-

100

Ammonium sulphate

5.5

60.3

0.332

30.2

0.166

1.996

1.406

60.62

Dialysis

5

41.21

0.206

8.7

0.0435

4.737

3.338

37.66

Concentrated

4.5

66.54

0.299

14

0.063

4.752

3.349

54.73

Gel filtration

5

51.82

0.259

10

0.05

5.18

3.65

47.34

Ion exchange DEAE-Cellulose A50 Isoenzyme-I

3

38.1

0.114

4.15

0.0124

9.209

6.490

20.87

Isoenzyme-II

3

16.363

0.049

2.2

0.0066

7.424

5.232

8.95