Primer | Sequence (5’ → 3’) | Purpose |
P1* | TNGCNGCNTTYGARTGGAAYGA | Primary PCR |
P2* | CATNCCNSWRAARTCRTCRTTRTC | Primary PCR |
P3* | GGNGGNTGGAAYATGGG | Primary PCR |
P4* | ACCCAYTGRTTNCCNARNACNA | Primary PCR |
P5* | GNAAYTTYGAYGGNYTNGA | Primary PCR |
P6* | TTDATCATYTCRCANACYTCRTARTA | Primary PCR |
P7 | GCCGGATACGAAGTGGAC | 3’ RACE |
P8 | GGAACTTAACGAGTACTT | 3’ RACE |
P9 | GACAGACGAGAGCGACTCTGGTCG | 3’ RACE |
3R | CTGTGAATGCTGCGACTACGAT | 3’ RACE |
P10 | CACAATGACGTTGCAAG | 5’ RACE, Full-length PCR |
P11 | ATGGCCTGGGCTCATTTT | 5’ RACE |
P12 | TTATCCTCTGGAGGGCT | 5’ RACE |
P13 | CACGTTATGATTGCGAC | Full-length PCR |
P14 | TCTGCTGCTGTGAGTGCTGGCAAGG | tissue-specific expression |
P15 | GCATTTCGCACACCTCGTAGTAAGA | tissue-specific expression |
β-actin-a* | GAYAAYGGNWSNGGNATGTG | tissue-specific expression |
β-actin-b* | TCRAACATDATYTGNGTCAT | tissue-specific expression |