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Types of extracts, elution systems and method of revelation used

Extract Types

Elution systems

Revelation/Observation

Flavonoids

Ethylacetate (P3) and aqueous (P5) extracts

Butanol/ AcOH/H₂O (12/1/1)

Neu,

Observation reagent at visible and UV 365 nm

Tannins

Ethylacetate extract (P3)

Butanol/ AcOH/H₂O (12/1/1)

2% FeCl₃

Visible observation

Coumarins

Hexane (P1), chloroform (P2), ethylacetate (P3) extracts

Toluene/ AcOEt/AcOH (1/1/1)

10% KOH in ethanol

Observation at UV 365 nm

Sugars

Ethanolic extract (P4)

Butanol/AcOH/AcOEt/H₂O (9/6/3/2)

Anisaldehyde + heating to 100˚C

Visible observation

Amino acids

Ethanolic extract (P4)

Butanol/AcOH/AcOEt/H₂O (9/6/3/2)

Ninhydrin + heating to 100˚C

Visible observation

Terpenes, Sterols and Carotenoids

Hexane (P1), chloroform (P2), ethylacetate (P3) extracts

Hexane/AcOEt (7/3)

Antimony chloride + heating to 110˚C

Visible observation