Horse qPCR method

Sample level (w/w)

0.1%

1.0%

10%

Number of data points

24

24

24

Mean estimated relative horse DNA content (%)

0.12

1.30

10.78

Relative repeatability standard deviation RSD_r (%)

22.06

6.18

3.22

Relative reproducibility standard deviation RSD_R (%)

46.04

19.43

10.90

Bias (absolute)

0.02

0.30

0.76

Bias (%)

15.27

30.32

7.75