Reagent Component

Laube Mammalian

Köppel Horse

2 × TaqMan Universal PCR Master Mix

1 ×

1 ×

Forward Primer

0.9 µM

0.3 µM

Reverse Primer

0.9 µM

0.3 µM

Probe

0.2 µM

0.2 µM

Water

Make up to 19 µL

Template DNA

6 µL volume

Test samples: 50 ng DNA

Standards: 130.25 ng to 0.05 ng DNA