Sampling No.	Sampling area			pH	NaCl (%)	Water temperature (˚C)	No. of isolation	Gas production
Sampling No.	Sampling area			pH	NaCl (%)	Water temperature (˚C)	No. of isolation	YPD	YPD + 30% Sorbitol	YPD + 10% Ethanol	YPD + 20% Sorbitol and 10% Ethanol	YPD + 30% Sorbitol and 10% Ethanol
Station 1	Tama River midstream	Asa River	Mud	8.19	0.00	26.5	217	18	16	1	1	1
Station 2		Hodokubo River	Mud	8.63	0.00	27.0	100	49	46	2	1	1
Station 3		Branch Junction	Mud	8.80	0.01	25.8	165	30	28	6	4	4
Stations 1 - 3							482	97	90	9	6	6
Station 4	Tama River downstream	Rokugo Bank	Mud	6.91	0.86	23.5	80	33	24	2	0	0
Station 5		Futakotama River.1	Mud	7.36	0.01	21.2	90	32	25	9	8	8
Station 6		Futakotama River.2	Mud	7.53	0.03	25.0	20	12	8	4	0	0
Stations 4 - 6							190	77	57	15	8	8
Station 7	Tama River estuary	Anamori Inari.1	Mud	7.93	0.37	27.0	51	37	33	7	6	4
Station 8		Anamori Inari.2	Mud	7.81	0.26	28.5	87	60	56	19	18	6
Station 9		Chidori Park	Water	8.49	3.20	28.5	33	16	16	4	4	0
Stations 7 - 9							171	113	105	30	28	10
Station 10	Lake Kasumigaura	Sakura River	Mud	7.74	0.00	21.5	64	61	61	4	3	1
Station 11		Nishiura	Mud	7.89	0.39	22.0	76	45	45	16	5	3
Station 12		Kitaura	Mud	8.81	0.02	22.0	45	19	19	9	4	3
Stations 10 - 12							185	125	125	29	12	7
Total							1028	412	377	83	54	31