Chemical Substances

No. of CFU

Mutant Frequency

4-NOPD Crystals (Positive Control)

30 - 38

2 - 2.4

Subtilosin

50 µg/mL

100 µg/mL

550 µg/mL

26

20

14

1.4

1.1

0.87

CFS of Bacillus subtilis KATMIRA1933

100%

50%

25%

12.5

14

14

11

15

0.87

0.9

0.7

1

CFS of Bacillus amyloliquefaciens B-1895

100%

50%

25%

12.5%

9

8

10

20

0.56

0.5

0.7

1.3

PBS (Negative Control)

18 - 20

1

MRS (Negative Control)

15 - 16

1

Disc only

0

0