| Bacterial Gene | Annealing Temp | Primer Sequences (5’ to 3’) | Product Size (bp) |
| pks island | 63˚C | F: TCGATATAGTCACGCCACCA | 733 |
| R: GTCAAGCGAGCATACGAACA | |||
| tcpC | 56˚C | F: TCGGCGATAGCTTAAGGAGA | 216 |
| R: CCGCCAAATAATGGCTGTAT | |||
| gelE [24] | 56˚C | F: TATGACAATGCTTTTTGGGAT | 213 |
| R: AGATGCACCCGAAATAATATA | |||
| cnf-1 | 56˚C | F: AGCGTGCAATCTATCCGTATTT | 173 |
| R: TGGAATTTCCCCAGTATAGGTG | |||
| cdt | 50˚C | F: AGATATCTTAATGATACAAGAAGCA | 196 |
| R: TGAAACTATAGCTAAATTTACACG |