Reference

PCR product (bp)

PCR conditions

Sequence (5′ → 3′)

Primer

6

This work

94˚C, 30 s; 55˚C, 30 s; 72˚C, 1 min (35 cycles)

AAA ACC GCC ACT GCG CCG TTA

IntI f

GAA GAC GGC TGC ACT GAA CG

IntI r

7

236

94˚C, 1 min; 48˚C, 45 s; 72˚C, 1 min (35 cycles)

ATCGCAATAGTTGGCGAAGT

qacED1-f

CAAGCTTTTGCCCATGAAGC

qacED1-r

7

437

94˚C, 1 min; 48˚C, 45 s; 72˚C, 1 min (35 cycles)

CTTCGATGAGAGCCGGCGGC

SulI-f

GCAAGGCGGAAACCCGCGCC

SulI-r

7

Variable

94˚C, 1 min; 48˚C, 45 s; 72˚C, 1 min (35 cycles)

GGCATCCAAGCAGCAAG

In5'CS

AAGCAGACTTGACCTGA

In3'CS