Column | Inertsil ODS-3, 150 × 4.6 mm 3 µ |
Column temperature | 35˚C |
Wave length | 270 nm for Imp-D and 340 nm for remaining impurities |
Injection volume | 100 µL |
Run time | 70 minutes |
Gradient | 90% A upto 8 min (isocratic) with 1.0 mL Linear gradient to 75% A at 20 min with 1.0 mL Linear gradient to 70% A at 45 min with 1.0 mL Linear gradient to 20% A at 50 min with 1.2 mL Linear gradient to 0% A at 52 min with 1.2 mL 0% A from 52 min to 60 min with 1.2 mL Linear gradient to 90% A at 62 min with 1.0 mL 90% A from 62 min to 70 min with 1.0 mL |