| Std Order | Run Order | Center Pt | Flow | pH | % MeOH | % ACN | Resolution b/w B & H |
| 1 | 1 | 1 | 0.8 | 2.6 | 35 | 35 | 1.765 |
| 2 | 2 | 1 | 1.2 | 2.6 | 35 | 45 | 2.951 |
| 3 | 3 | 1 | 0.8 | 3.0 | 35 | 45 | 1.774 |
| 4 | 4 | 1 | 1.2 | 3.0 | 35 | 35 | 2.613 |
| 5 | 5 | 1 | 0.8 | 2.6 | 45 | 45 | 1.806 |
| 6 | 6 | 1 | 1.2 | 2.6 | 45 | 35 | 2.706 |
| 7 | 7 | 1 | 0.8 | 3.0 | 45 | 35 | 1.611 |
| 8 | 8 | 1 | 1.2 | 3.0 | 45 | 45 | 2.813 |
| 9 | 9 | 0 | 1.0 | 2.8 | 40 | 40 | 2.232 |
| 10 | 10 | 0 | 1.0 | 2.8 | 40 | 40 | 2.212 |