Parameter

2-hydroxy-5-methyl pyridine

Pirfenidone

Iodobenzene

0.8 ml/min

2.24

1.75

0.95

1.0 ml/min

1.50

1.24

1.36

1.2 ml/min

1.96

1.68

0.5

pH 2.3

0.67

1.51

0.82

pH 2.5

1.50

1.24

1.36

pH 2.7

3.80

1.32

2.48

Buffer strength 0.015 M

1.68

3.16

1.54

Buffer strength 0.020 M

1.50

1.24

1.36

Buffer strength 0.025 M

0.43

1.55

1.55

Injection volume 10 µL

1.00

2.18

3.92

Injection volume 20 µL

1.50

1.24

1.36

Injection volume 30 µL

2.66

1.89

0.82

Gradient program 63:37 v/v

0.50

3.38

0.93

Gradient program 65:35 v/v

1.50

1.24

1.36

Gradient program 73:33 v/v

0.84

2.07

1.55