Validation parameter

% RSD of peak area in standard solution for five replicate injections (NMT 5.0%)

2-hydroxy-5-methylpyridine

Pirfenidone

Iodobenzene

Specificity

1.84

1.54

0.85

Determination of LOD & LOQ values

0.80

1.62

1.80

Precision at LOQ

1.28

1.31

1.93

Linearity study

1.62

2.11

1.20

Accuracy

1.80

1.11

0.56

System precision

1.94

0.81

2.26

Method precision

1.91

1.54

2.99

Intermediate precision

0.52

2.92

1.83

Robustness study-actual

1.30

1.77

1.95