| Optimal condition by group | Final optimal conditions | ||||||
| Optimal conditions | Optimal P (S > 0) | Flow rate (F in mL/min) | Organic modifier (OM in %) | Temperature (T in ˚C) | |||
| Groups | Subgroups | ||||||
| 1 | - | 68.0% | 0.45 (0.45 - 0.61) | 81.3 (80.0 - 82.1) | 25.0 (25.0 - 35.0) | OM: 80.0% F: 0.5 mL/min T: 25˚C | |
| 2 | - | 99.5% | 0.70 (0.55 - 0.70) | 80.0 (80.0 - 81.8) | 32.5 (25.0 - 34.5) | ||
| 3 | - | 73.0% | 0.45 (0.41 - 6.50) | 81.3 (81.0 - 82.1) | 25.0 (25.0 - 27.0) | ||
| 4 | - | 92.9% | 0.65 (0.48 - 7.00) | 80.0 (80.0 - 81.5) | 32.5 (25.0 - 35.0) | ||
| 5 | 1 | 98.5% | 0.70 (0.61 - 0.70) | 81.3 (80.5 - 82.0) | 26.3 (25.0 - 35.0) | OM: 82.5% F: 0.6 mL/min T: 25˚C | |
| 2 | 99.9% | 0.40 (0.55 - 0.70) | 88.8 (87.5 - 90.0) | 25.0 (25.0 - 35.0) | |||