| S/NO | Test | Reagent | PEE | EAE | MEE | AWE |
| 1 | Alkaloid | Dragendorff’s and Mayer’s Reagents | − | + | + | − |
| 2 | Saponins | NaHC03aq Emulsion foaming test | − | + | + | − |
| 3 | Tannins | 5% FeCl3aq and 10% KOHaq | − | − | + | + |
| 4 | Flavonoids | Dil. Ammonia andconc.H2SO4 | − | + | + | + |
| 5 | Anthraquinones | H2SO4, Chloroform and Ammonia | + | − | + | + |