| S/NO | TEST | OBSERVATION | INFERENCE |
| 1 | Draggen dorff’s and Mayer’s reagents | No turbidity or precipitation | Alkaloids absent |
| 2 | Frothing test | Frothing did not persist on warming | Saponins absent |
| 3 | Ferric chloride reagent test | No blue-green precipitate formed | Tannins absent |
| 4 | Dil. Ammonia and conc. H2So4 test | No yellow colouration formed on standing | Flavonoids absent |
| 5. | H2SO4, chloroform and Ammonia test | The resulting solution changes from brown to colourless | Anthraquinones present |