(NH4)₂SO₄^a conc^b

Elution with

Protein conc. of E. histolytica

40%

0.25M NaCl^c

1.0 mg/mL

40%

0.5M NaCl

0.75 mg/mL

60%

0.25M NaCl

0.8 mg/mL

60%

0.5M NaCl

0.9 mg/mL

80%

0.25M NaCl

0.7 mg/mL

80%

0.5M NaCl

1.5 mg/mL

100%

0.25M NaCl

0.2 mg/mL

100%

0.5M NaCl

0.1 mg/mL