Treatment

amendment

16S

18S

ureC

phoA

cbbLR

18S/16S

ureC/16S

phoA/16S

cbblR/16S

Treatment Combinations

Normalized Ratio (gene/16S) Probability

Control

Control

6.3 × 1011

3.06 × 1011

1.7 × 108

8.64 × 105

3.93 × 107

BC or L

*

Poultry litter (PL)

Control

7.08 × 1011

8.72 × 1011

3.44 × 108

6.73 × 105

6.75 × 107

PL, PL + BC, and PL + L or Control

*

Fertilizer (FRT)

Control

7.62 × 1011

3.7 × 1011

2.77 × 108

8.73 × 105

5.00 × 107

PL or all other treatments

*

Poultry litter (PL)

Biochar (BC)

6.49 × 1011

4.28 × 1011

2.72 × 108

9.18 × 105

4.11 × 107

PL, PL + BC, and PL + L or all other treatments

*

Poultry litter (PL)

Lignite (L)

5.86 × 1011

4.35 × 1011

2.37 × 108

1.19 × 106

3.98 × 107

Fertilizer (FRT)

Biochar (BC)

6.83 × 1011

4.25 × 1011

2.55 × 108

2.74 × 106

4.72 × 107

Fertilizer (FRT)

Lignite (L)

7.53 × 1011

3.17 × 1011

3.13 × 108

9.4 × 105

4.33 × 107

PL + BC + L

6.74 × 1011

7.62 × 1011

2.72 × 108

2.64 × 106

5.03 × 107