| Group | Saline | β-glucan | Total dose |
| Control | 0.50 ml | 0 | 0.50 ml |
| Group A | 0.375 ml | 0.125 ml | 0.50 ml |
| Group B | 0.25 ml | 0.25 ml | 0.50 ml |
| Group C | 0 | 0.50 ml | 0.50 ml |