Primer

Tm

Sequence

Specificity

FUT2-Se-428-s

68.8˚C

5’-CCGGTACCCCTGCTCGTG-3’

Se (direct)

FUT2-se-428-f

66.6˚C

5’-ACCGGTACCCCTGCTCGTA-3’

se (direct)

FUT2-all-523-as

66.7˚C

5’-CCGGCTCCCGTTCACCTG-3’

No specífic (reverse)