Treget gene

Primer sequence

Annealing temperature

DR4

Forward: 5’-TCCAGCAAATGGTGCTGAC-3’

Reverse: 5’-GAGTCAAAGGGCACGATGTT-3’

60˚C

DR5

Forward: 5’-CCAGCAAATGAAGGTGATCC-3’

Reverse: 5’-GCACCAAGTCTGCAAAGTCA-3’

61˚C

DcR1

Forward: 5’-TCCTGCTGCCAGTCCTAGCTTAC-3

Reverse: 5’-TGAGATCCTGCTGGACACTCCTC-3’

61˚C

DcR2

Forward: 5’-GCCGGTCCGGGTTGACTC-3’

Reverse: 5’-TGAGATCCTGCTGGACACTCCT-3’

60˚C

GADPH

Forward: 5’-TGCACCACCAACTGCTTAG-3’

Reverse: 5’-GGATGCAGGGATGATGTT-3’

58˚C