Levels

Factors

A (glucose, g/L)

B (bean pulp, g/L)

C (K₂HPO₄, g/L)

Medium optimization orthogonal test

1

30

40

1.0

2

20

35

2.0

3

15

30

2.5

Levels

Factors

A (inoculum size, %)

B (initial pH)

C (loading volume, mL/250 mL)

Fermentation condition optimization orthogonal test

1

2

7

20

2

3

7.5

30

3

4

8

40