Number of cycles

Step

Temperature, ˚C

Duration

Cycle 1: (1×)

Step 1

96.0

1 min

Cycle 2: (5×)

Step 1

96.0

20 sec

Step 2

70.0

45 sec

Step 3

72.0

25 sec

Cycle 3: (21×)

Step 1

96.0

25 sec

Step 2

65.0

50 sec

Step 3

72.0

30 sec

Cycle 4: (4×)

Step 1

96.0

30 sec

Step 2

55.0

1 min

Step 3

72.0

1 min 30 sec

Cycle 5: (1×)

Step 1

4.0

∞