Plant growth regulator (mg/L)

Day of callus formation

Explant producing callus (%)

Callus proliferation (%)

Morphology of callus

2,4-D

BAP

1.0

1

-

0

0

3

-

0

0

5

-

0

0

5.0

1

-

0

0

3

-

0

0

5

-

0

0

10.0

1

3 - 6

2.4

0

All calluses that obtained were compact, nodular, yellowish, brown in colour. The callus turned browning after 9 months of culture, and no proliferation occurred

3

3 - 6

0.5

0

5

-

0

0

20.1

1

3 - 6

0.8

0

3

3 - 6

0.1

0

5

-

0

0

40.1

1

3 - 6

0.4

0

3

3 - 6

0.1

0

5

-

0

0

60.0

1

-

0

0

3

-

0

0

5

-

0

0

100.0

1

-

0

0

3

-

0

0

5

-

0

0