Plant growth regulator (mg/L)

Day of callus formation

Explant producing callus (%)

Callus proliferation (%)

Morphology of callus

2,4-D

NAA

1.0

1

-

0

0

3

-

0

0

5

-

0

0

5.0

1

-

0

0

3

-

0

0

5

-

0

0

10.0

1

2 - 3

2.7

0

All calluses that obtained were compact, translucent and yellowish in colour. Some adventitious roots were developed. The callus turned browning after 5 months of culture, and no proliferation occurred

3

2 - 3

1.5

0

5

-

0

0

20.1

1

2 - 3

0.7

0

3

2 - 3

0.4

0

5

-

0

0

40.1

1

2 - 3

0.5

0

3

-

0

0

5

-

0

0

60.0

1

3 - 5

0.1

3

-

0

0

5

-

0

0

100.0

1

-

0

0

3

-

0

0

5

-

0

0