Plant growth regulator

2,4-D (mg/L)

Day of callus formation

(months)

Explant producing callus (%)

Callus proliferation (%)

Morphology of callus

1

-

0

0

Early stage initiation, all calluses were translucent.

After 3 - 5 months, callus turned friable and yellowish.

5

-

0

0

10

7 - 9

0.5

3

20

7 - 9

0.7

3

40

11 - 12

2.9

5

60

11 - 12

1.5.0

8

100

11 - 12

1.0

20