Source organism

Thermobifida alba

DNA source

Genbank AB445476

Forward primer

5’- CGCGGATCCAACCCCTACGAACGCGGC -3’

Reverse primer

5’- GCGAAGCTTGAACGGGCAGGTGGAGCGGT -3’

Expression vector

pQE80L- est1(A68V/T253P)

Expression host

E. coli Rosetta-gami (DE3)

Complete amino acid sequence of

the construct produced

MRGSHHHHHHGSNPYERGPNPTESMLEARSGPFSVS

EERVSRLGADGFGGGTIYYPRENNTYGAIAISPGYTG

TQSSIAWLGERIASHGFVVIAIDTNTTLDQPDSRARQL

NAALDYMLTDASSSVRNRIDASRLAVMGHSMGGGG

TLRLASQRPDLKAAIPLTPWHLNKSWRDITVPTLIIGA

DLDTIAPVSSHSEPFYNSIPSSTDKAYLELNNATHFAPN

IPNKTIGMYSVAWLKRFVDEDTRYTQFLCPGPRTGLLS

DVDEYRSTCPF