| Primer Name | Sequences | Size (bp) | Uses |
| DOT1L-P1R | GGA GAC A/ideoxyU/GTTCGCGGCTTAACCCCCGAAA | 1079 | Left arm |
| DOT1L-P1F | GGG AAA G/ideoxyU/TGCACGGTGGCGAACTCCAG |
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| DOT1L-P2R | GGC ATA G/ideoxyU/TCCTAAGAGACCCAGCATAG | 1152 | Right arm |
| DOT1L-P2F | GGTCCCA/ideoxyU/AGACCTTGCTTAGCTAGCAG |
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| F1 | CAGGTTCCCTTCCGCACTCT | 1713 | picking positive colony |
| NR | GTTGTGCCCAGTCATAGCCG |
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| NF | TCTGGATTCATCGACTGTGG | 1750 | picking positive colony |
| R1 | TAGTTACCTGCAGAAGGGCA |
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| F2 | CCGCCTGCTAACGCCTCTTT | 649 | Surveyor nuclease cutting |
| R2 | ACGCCACCCGTCATGAGTGA |
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