| Run | Block | Coded value | Mycelium mass (g) | |||
| Agitation rate | Temp | pH | Response | Predicted value | ||
| 1 | 1 | 0 | 0 | 0 | 0.77 | 0.93 |
| 2 | 1 | −1 | 0 | 0 | 0.35 | 0.49 |
| 3 | 1 | 1 | −1 | 1 | 1.74 | 1.68 |
| 4 | 1 | −1 | 1 | −1 | 0.19 | 0.25 |
| 5 | 1 | 0 | 1 | 0 | 1.29 | 1.07 |
| 6 | 1 | −1 | −1 | −1 | 0.07 | −0.09 |
| 7 | 1 | −1 | −1 | 1 | 0.21 | 0.18 |
| 8 | 1 | 1 | 1 | −1 | 2.54 | 2.56 |
| 9 | 1 | 0 | 0 | 0 | 0.74 | 0.93 |
| 10 | 1 | 0 | 0 | −1 | 0.63 | 0.71 |
| 11 | 1 | 0 | 0 | 0 | 0.84 | 0.93 |
| 12 | 1 | 1 | −1 | −1 | 1.56 | 1.56 |
| 13 | 1 | 0 | 0 | 0 | 0.94 | 0.93 |
| 14 | 1 | 1 | 0 | 0 | 2.5 | 2.4 |
| 15 | 1 | −1 | 1 | 1 | 0.4 | 0.39 |
| 16 | 1 | 1 | 1 | 1 | 2.41 | 2.56 |
| 17 | 1 | 0 | 0 | 0 | 1.08 | 0.93 |
| 18 | 1 | 0 | 0 | 1 | 0.89 | 0.84 |
| 19 | 1 | 0 | −1 | 0 | 0.21 | 0.18 |
| 20 | 1 | 0 | 0 | 0 | 1.25 | 0.93 |