87

Vigneswaran et al. [66]

Asphergillus flavus fungal cells

1 mM, 24 hr, 37˚C,

Dark. 5 g/100 ml,

Shaken

UV-Vis

TEM

XRD

FTIR

FS

Size―8.92 ± 1.61 nm

Shape―Isotropic

Structure―FCC

Nature―MD

AgNP stable for 3 months

88

Bhainsa and D’souza (2006) [54]

Aspherillus fumigates aqueous cell filtrate

1 mM, 1 hr, 25˚C,

Dark,

Shaken

UV―Vis

TEM

XRD

Size―5 - 25 nm

Shape―Sph and Tri.

Structure―Crystal

Nature―WD

No precipitation of AgNP observed upto 72 hrs

89

Vigneswaran et al. [67]

Phaenerochaete

chrysosporium mycelium

1 mM, 24 hr, 37˚C,

Dark,

Shaken

UV-Vis

XRD

SEM

TEM

FS

Size―50 - 200 nm Shape―sph. and hex.

Structure―FCC

Nature―non uniform

AgNP formed on the surface of mycelium

90

Duran et al (2005) [56]

Aqueous filtrate and biomass of Fusarium oxysporum species.

1 mM, 28 hr, 28˚C.

10 g/100 ml

Static.

UV-Vis

SEM

Size―20 - 50 nm

Shape―sph.

Nitrate based reductase promoted the AgNP synthesis

91

Senapati et al. (2004) [133]

Verticillium and F. oxysporum

UV-Vis

SEM/TEM

Size―Verticillium 25 ± 8 nm, F. oxysporum―5 - 50 nm

Verticillium (intracellular) and F. oxysporum―extracellular synthesis.

92

Ahmad et al. (2003) [55]

Fusarium oxysporum biomass

1 mM, 72 hr, room temp, dark 10 g/100 ml,

Static

UV-Vis

XRD

TEM

FTIR

FS

Size―5 - 50 nm

Shape―sph/tri.

Structure―FCC

93

Mukherjee et al. (2001) [52]

Harvested mycelia of Verticillium sp. fungi

0.2 mM, 72 hr, 28˚C,

10 g/100 ml,

Shaken,

pH: 5.5 - 6

UV-Vis

SEM

TEM

EDAX

Size―25 ± 12 nm

Shape―nearly sph, Nature― monodispersed

AgNPs were synthesized on intracellular bases.

Gram positive Bacteria

94

Zhang et al.

(2014) [134]

Lactobacillus fermentum.LMG 8900 cells

10 g/L, 24 hr, 30˚C,

10 g/L, Shaken,

6 min at 5000 rpm and 10 min at 6000 rpm

UV-Vis

TEM

XRD

ZP

Size―~6 nm

Shape―sph.

Structure―FCC

Stable for 3 months.

Resist growth of E. coli, S. aureus and P. aeruginosa

Act as promising anti-biofouling agent

95

Zonnoz and Salouti (2011) [83]

Aqueous cell filtrate of Streptomyces sp. ERI-3

1 mM, 48 hr, 28˚C.

Dark.

Shaken.

UV-Vis

XRD

TEM

SEM

Size―10 - 100 nm

Shape―Spherical

After 3 months, nanoparticles developed floret shape

96

Deepak et al.

(2011) [135]

Fibrinolytic URAK enzyme produced by Bacillus cereus NK1

1 mM, 24 hr without NaOH and 5 min with NaOH, 37˚C,

URAK content: 1 mg, additives: 10 ml of Tris-Hcl buffer of pH 9

UV-Vis

TEM

XRD

AFM

Size―50 - 80 nm

Shape―sph.

Structure―FCC

Nature―WD

AgNP with mmobilized enzyme

97

Kalishwarlal et al (2010) [136]

Brevibacterium casei harvested cells

1 mM, 24 hr, 37˚C, 1 g, Shaken,

30 min at 16000 g

UV-Vis

TEM

XRD

FTIR

FS

Size―10 - 50 nm.

Shape―Sph.

Structure―FCC

AgNP act as stable anti-coagulant

98

Ganeshbabu and Gunasekaran (2009) [137]

Isolated and harvested Bacillus cereus PGN1 cells.

1 mM, 120 hr, 37˚C.

10 g/100 ml.

15 min at 15000 rpm.

Shaken.

UV-Vis

FTIR

XRD

TEM

Size-4-5 nm

Shape-Sph.

Structure-FCC.

Nature-MD.

Tris Buffer (pH-7) as suspension media for nanoparticles

99

Nanda et al (2009) [138]

Staphylococcus aureus supernatant

1 mM, 5 min

UV-Vis

AFM

Size―160 - 180 nm

Nature―PD.

AgNP antibacterial action against human pathogenic bacteria MRSA, MRSE, S. pyogenes

100

Kalimuthu et al (2008) [139]

Bacillus icheniormis cells

1 mM, 24 hr, 37˚C,

30 min at 15000 rpm.

Shaken

UV-Vis

SEM

EDX

XRD

Size―50 nm

Structure―Crystal

Nature―WD

Gram negative bacteria

101

Perni et al

(2013) [58]

Escherichia coli cells

1 or 5 mM, 24 hr, 30˚C,

Ratio of AgNO3: L-cysteine = 1:5,

Shaken,

10 min at 1851 g

UV-Vis

FTIR

TEM

TGA

Size―~5 nm

Capping agent: L-cysteine,

Antimicrobial against E. coliand S. aureus

102

Juibari et al. (2011) [140]

Ureibacillus thermo sphaerius supernatant

1 - 100 mM, 24 hr, 60˚C - 80˚C,

Dark, 15 min (13 k, rpm Static

UV-Vis

DLS

XRD

FTIR

TEM

Size―10 - 100 nm

Shape―Sph.

Structure―FCC

Nature―PD

Temperature around 80˚C stands possible because of thermophilic nature of bacteria

103

Gurunathan et al. (2009) [141]

E. coli supernatant

1 - 10 mM, 24 hr, 20˚C - 90˚C,

pH: 5 - 12,

10 min at 10k rpm

Static

UV-Vis

DLS

TEM

FTIR

Size―10 - 90 nm

Shape―Sph.

Structure―Crystal

Nature―Uniform

Nitrate medium (pH-8) is used for culture.

104

Shahverdi et al (2007) [59]

K. pneumonia (Enterobacteria) supernatant

1 mM, 5 min, Room temp.

UV-Vis

TEM

EDS

Size―Avg: 52.25 nm

Shape―Sph.

Nanoparticles are unstable after 5 min. Addition of piperitone resisted the nanoparticle growth.

Bio-polymers