Lipase sources

Substrate

Vmax

(mM·min⁻¹·mg⁻¹)

Km

(mM)

Kcat

(s⁻¹)

Kcat/Km

(mM⁻¹·s⁻¹)

G. stearothermophilus

PNPC2

22 ± 2.6

64 ± 10

9.3 ± 1.1 × 10⁸

1.5 ± 0.1 × 10¹⁰

PNPC8

2.3 ± 0.3

14.6 ± 2

9.7 ± 0.6 × 10⁷

6.7 ± 1.0 × 10¹⁰

PNPC12

2.6 ± 0.4

0.8 ± 0.0

1.1 ± 0.2 × 10⁸

1.4 ± 0.3 × 10¹¹

PNPC14

2 ± 0.1

2.3 ± 0.1

8.3 ± 1.4 × 10⁷

3.6 ± 0.3 × 10¹⁰

PNPC16

2 ± 0.0

1.9 ± 0.2

8.6 ± 0.8 × 10⁷

4.7 ± 0.1 × 10¹⁰

A. flavithermus

PNPC2

1.1 ± 0.1

2.4 ± 0.1

3.5 ± 0.5 × 10⁷

1.5 ± 0.0 × 10¹⁰

PNPC8

1.4 ± 0.2

0.4 ± 0.0

4.6 ± 0.8 × 10⁷

1.1 ± 0.0 × 10¹¹

PNPC12

0.9 ± 0.1

1.1 ± 0.1

3.1 ± 0.6 × 10⁷

2.7 ± 0.2 × 10¹⁰

PNPC14

2.5 ± 0.2

6.6 ± 0.5

8.2 ± 0.9 × 10⁷

1.2 ± 0.2 × 10¹⁰

PNPC16

1.1 ± 0.0

1.5 ± 0.4

3.5 ± 0.3 × 10⁷

2.3 ± 0.9 × 10¹⁰