Analysis

Method

Moisture (water)

AOAC, 2000. Official method of analysis 934.01^*

Ash

AOAC, 2000. Official method of analysis 942.05^*

Protein (N)

AOAC, 2000. Official method of analysis 968.06^*

Fat

AOAC, 2000. Official method of analysis 920.39^*

Energy (calculated)

Greenfield, H. and Southgate, D.A.T., 2003^#

Carbohydrates (calculated)

Greenfield, H. and Southgate, D.A.T., 2003^#

Available carbohydrates

Available carbohydrates = Dry Matter ? Protein ? Fat ? Ash ? NDF (neutral detergent fibre) (Ferreira, 2014)

Starch

Enzymatic spectrophotometer (In-house method)

Minerals

AOAC, 2000. Official method of analysis 935.13^* [32]