Primer name

Primer sequence (5’-3’)

Usage

E3 upl-F

CGACAAGAAGGCGATGGCGAC

PCR

E3 upl-R

TCTTCATCATTAGCAATATGCATTCC

E3 upl-F2

GATATCGACAAGAAGGCGATGGCGAC (EcoR V)

pET32a

E3 upl-R2

GAATTCTCAGGGATCAGCAGAAGCAGG (EcoR I)