Herbal preparation | Pumpkin seed oil | Pumpkin seed soft extract | Δ7-sterols from pumpkin seed oil | |
Latin binominal name | Company-owned variety of Cucurbita pepo L. convar. citrullinina GREB. var. styriaca GREB | |||
Family name | Cucurbitaceae | |||
Common names | Uromedic® pumpkin | |||
Plant part | Seed | |||
Country of origin of herbal raw material | Hungary | |||
Voucher specimen numbers | Batch number of commercial Herbal Substance (corresponding to drug substance batch below) | Material 3000000293 | Material 3000000299 | Commercially not available |
Batch number of commercial drug substance (used for this study) | Omega Pharma batch: 511062, vendor/batch: 603827/283484 | Omega Pharma batch: 531057, vendor/batch: 600516/15000533 | Commercially not available | |
Authorized drug substance manufacturer | Estyria Naturprodukte GmbH; St. Ruprecht/Raab, Austria | Finzelberg GmbH & Co. KG; Andernach, Germany | not applicable | |
Further extraction | none | none | Isolation of Δ7-sterols at the Department of Pharmacy, Ludwig-Maximilians-University; Munich, Germany | |
Test substance used for in vitro experiments | Oil | Soft extract | Isolated Δ7-sterols | |
Type and concentration of extraction solvent used; drug-extract ratio (DER) | not applicable: pure oil without additives | Extraction solvent: ethanol 92% (m/m), DER 15 - 25:1 | not applicable | |
Content of quantified herbal constituents/purity | 100% oil, Ph. Eur. | 95% - 99% native extract | not applicable, since no extract: isolated Δ7-sterols | |
Contained excipients | none | 1-5% hydrophobic colloidal silica | none | |
Identification | Fatty acid profile (TLC) | Phytosterols (GC) | Δ7-sterols (GC-IT-MS) | |
Typical marker compounds | Phytosterols, Fatty acid profile | Phytosterols | Δ7-sterols (e.g. Δ7,25-stigmasterol, spinasterol, Δ7-avenasterol) |