Herbal preparation

Pumpkin seed oil

Pumpkin seed soft extract

Δ7-sterols from pumpkin seed oil

Latin binominal name

Company-owned variety of Cucurbita pepo L. convar. citrullinina GREB. var. styriaca GREB

Family name


Common names

Uromedic® pumpkin

Plant part


Country of origin of herbal raw material


Voucher specimen numbers

Batch number of commercial Herbal Substance (corresponding to drug substance batch below)

Material 3000000293

Material 3000000299

Commercially not available

Batch number of commercial drug substance (used for this study)

Omega Pharma batch: 511062, vendor/batch: 603827/283484

Omega Pharma batch: 531057, vendor/batch: 600516/15000533

Commercially not available

Authorized drug substance manufacturer

Estyria Naturprodukte GmbH; St. Ruprecht/Raab, Austria

Finzelberg GmbH & Co. KG; Andernach, Germany

not applicable

Further extraction



Isolation of Δ7-sterols at the Department of Pharmacy, Ludwig-Maximilians-University; Munich, Germany

Test substance used for in vitro experiments


Soft extract

Isolated Δ7-sterols

Type and concentration of extraction solvent used; drug-extract ratio (DER)

not applicable: pure oil without additives

Extraction solvent: ethanol 92% (m/m), DER 15 - 25:1

not applicable

Content of quantified herbal constituents/purity

100% oil, Ph. Eur.

95% - 99% native extract

not applicable, since no extract: isolated Δ7-sterols

Contained excipients


1-5% hydrophobic colloidal silica



Fatty acid profile (TLC)

Phytosterols (GC)

Δ7-sterols (GC-IT-MS)

Typical marker compounds

Phytosterols, Fatty acid profile


Δ7-sterols (e.g. Δ7,25-stigmasterol, spinasterol, Δ7-avenasterol)