Gene

Primer Sequence

TWIST

Methylation forward; 5'-TTTCGGATGGGGTTGTTATCG-3'

Methylation reverse; 5'-GACGAACGCGAAACGATTTC-3'

Unmethylation forward; 5'-TTGGATGGGGTTGTTATTGT-3'

Unmethylation reverse; 5'-ACCTTCCTCCAACAAACACA-3'