PCR

Genes Cibles

Sequences Amorces (5'-3')

Size of the Amplicon (pb)

Sources

Search for integrons

intI1

F: 5'-GCCTTGATGTTACCCGAGAG-3'

196

[21]

R: 5'-GATCGGTCGAATGCGTGT-3'

PCR Cycle: 95˚C/3min; (95˚C/30s, 60˚C/30s, 72˚C/30s) × 35; 72˚C/7min

intI2

F: 5'-TGCTTTTCCCACCCTTACC-3'

195

[21]

R: 5'-GACGGCTACCCTCTGTTATCTC-3'

PCR Cycle: 95˚C/3min; (95˚C/30s, 62˚C/30s, 72˚C/30s) × 35; 72˚C/7min

intI3

F: 5'-GCCACCACTTGTTTGAGGA-3'

138

[21]

R: 5'-GGATGTCTGTGCCTGCTTG-3'

PCR Cycle: 95˚C/3min; (95˚C/30s, 62˚C/30s, 72˚C/30s) × 35; 72˚C/7min

Searching for genes encoding ESBL

blaTEM

F: 5'-GCKGCCAACTTACTTCTGACAACG-3'

247

[22]

R: 5'-CTTTATCCGCCTCCATCCAGTCTA-3'

PCR Cycle: 95˚C/3min; (95˚C/30s, 60˚C/30s, 72˚C/30s) × 35; 72˚C/7min

blaSHV

F: 5'-CGCTTTCCCATGATGAGCACCTTT-3'

110

[22]

R: 5'-TCCTGCTGGCGATAGTGGATCTTT-3'

PCR Cycle: 95˚C/3min; (95˚C/30s, 60˚C/30s, 72˚C/30s) × 35; 72˚C/7min

blaCTX-M

F: 5'-CTATGGCACCACCAACGATA-3'

103

[23]

R: 5'-ACGGCTTTCTGCCTTAGGTT-3'

PCR Cycle: 95˚C/3min; (95˚C/30s, 62˚C/30s, 72˚C/30s) × 35; 72˚C/7min

Search for carbapenemase coding genes

blaNDM

F: 5'-GATTGCGACTTATGCCAATG-3'

189

[24]

R: 5'-TCGATCCCAACGGTGATATT-3'

PCR Cycle: 95˚C/3min; (95˚C/30s, 60˚C/30s, 72˚C/30s) × 35; 72˚C/7min

blaKPC

F: 5'-CAGCTCATTCAAGGGCTTTC-3'

196

[24]

R: 5'-GGCGGCGTTATCACTGTATT-3'

PCR Cycle: 95˚C/3min; (95˚C/30s, 62˚C/30s, 72˚C/30s) × 35; 72˚C/7min

blaIMP

F: 5'-GGAATAGAGTGGCTTAAYTCTC-3'

200

[24]

R: 5'-GGTTTAAYAAAACAACCACC-3'

PCR Cycle: 95˚C/3min; (95˚C/30s, 56˚C/30s, 72˚C/30s) × 35; 72˚C/7min

blaVIM

F: 5'-GATGGTGTTTGGTCGCATA-3'

390

[24]

R: 5'-CGAATGCGCAGCACCAG-3'

PCR Cycle: 94˚C/2min; (94˚C/1min, 58˚C/30s, 72˚C/1min) × 35; 72˚C/7min

blaOXA48

F: 5'-AGGCACGTATGAGCAAGATG-3'

189

[24]

R: 5'-TGGCTTGTTTGACAATACGC-3'

PCR Cycle: 95˚C/3min; (95˚C/30s, 60˚C/30s, 72˚C/30s) × 35; 72˚C/7min