PCR | Genes Cibles | Sequences Amorces (5'-3') | Size of the Amplicon (pb) | Sources |
Search for integrons | intI1 | F: 5'-GCCTTGATGTTACCCGAGAG-3' | 196 | [21] |
R: 5'-GATCGGTCGAATGCGTGT-3' | ||||
PCR Cycle: 95˚C/3min; (95˚C/30s, 60˚C/30s, 72˚C/30s) × 35; 72˚C/7min | ||||
intI2 | F: 5'-TGCTTTTCCCACCCTTACC-3' | 195 | [21] | |
R: 5'-GACGGCTACCCTCTGTTATCTC-3' | ||||
PCR Cycle: 95˚C/3min; (95˚C/30s, 62˚C/30s, 72˚C/30s) × 35; 72˚C/7min | ||||
intI3 | F: 5'-GCCACCACTTGTTTGAGGA-3' | 138 | [21] | |
R: 5'-GGATGTCTGTGCCTGCTTG-3' | ||||
PCR Cycle: 95˚C/3min; (95˚C/30s, 62˚C/30s, 72˚C/30s) × 35; 72˚C/7min | ||||
Searching for genes encoding ESBL | blaTEM | F: 5'-GCKGCCAACTTACTTCTGACAACG-3' | 247 | [22] |
R: 5'-CTTTATCCGCCTCCATCCAGTCTA-3' | ||||
PCR Cycle: 95˚C/3min; (95˚C/30s, 60˚C/30s, 72˚C/30s) × 35; 72˚C/7min | ||||
blaSHV | F: 5'-CGCTTTCCCATGATGAGCACCTTT-3' | 110 | [22] | |
R: 5'-TCCTGCTGGCGATAGTGGATCTTT-3' | ||||
PCR Cycle: 95˚C/3min; (95˚C/30s, 60˚C/30s, 72˚C/30s) × 35; 72˚C/7min | ||||
blaCTX-M | F: 5'-CTATGGCACCACCAACGATA-3' | 103 | [23] | |
R: 5'-ACGGCTTTCTGCCTTAGGTT-3' | ||||
PCR Cycle: 95˚C/3min; (95˚C/30s, 62˚C/30s, 72˚C/30s) × 35; 72˚C/7min | ||||
Search for carbapenemase coding genes | blaNDM | F: 5'-GATTGCGACTTATGCCAATG-3' | 189 | [24] |
R: 5'-TCGATCCCAACGGTGATATT-3' | ||||
PCR Cycle: 95˚C/3min; (95˚C/30s, 60˚C/30s, 72˚C/30s) × 35; 72˚C/7min | ||||
blaKPC | F: 5'-CAGCTCATTCAAGGGCTTTC-3' | 196 | [24] | |
R: 5'-GGCGGCGTTATCACTGTATT-3' | ||||
PCR Cycle: 95˚C/3min; (95˚C/30s, 62˚C/30s, 72˚C/30s) × 35; 72˚C/7min | ||||
blaIMP | F: 5'-GGAATAGAGTGGCTTAAYTCTC-3' | 200 | [24] | |
R: 5'-GGTTTAAYAAAACAACCACC-3' | ||||
PCR Cycle: 95˚C/3min; (95˚C/30s, 56˚C/30s, 72˚C/30s) × 35; 72˚C/7min | ||||
blaVIM | F: 5'-GATGGTGTTTGGTCGCATA-3' | 390 | [24] | |
R: 5'-CGAATGCGCAGCACCAG-3' | ||||
PCR Cycle: 94˚C/2min; (94˚C/1min, 58˚C/30s, 72˚C/1min) × 35; 72˚C/7min | ||||
blaOXA48 | F: 5'-AGGCACGTATGAGCAAGATG-3' | 189 | [24] | |
R: 5'-TGGCTTGTTTGACAATACGC-3' | ||||
PCR Cycle: 95˚C/3min; (95˚C/30s, 60˚C/30s, 72˚C/30s) × 35; 72˚C/7min |