Sequencing Mix | x1 volum (µL) | x100 |
5X Sequencing Buffer | 4 | 400 |
PCR Water | 14.75 | 1475 |
Terminator nucleotides + Polymerase | 1 | 100 |
Primer Forward (20 µM/pmol) | 0.25 | 25 |
Primer Reverse (20 µM/pmol) | 0.25 | 25 |
Purified DNA | 0.5 |
|
Final volum | 20.5 |
|