Sequencing Mix

x1 volum (µL)

x100

5X Sequencing Buffer

4

400

PCR Water

14.75

1475

Terminator nucleotides + Polymerase

1

100

Primer Forward (20 µM/pmol)

0.25

25

Primer Reverse (20 µM/pmol)

0.25

25

Purified DNA

0.5

Final volum

20.5